Open AccessChemiluminescence Immunoassay to Detect Anti‐HTLV‐I Antibodies
Author(s) -
Kamihira Shimeru,
Nakashima Shigehiro,
Saitoh Shin,
Kawamoto Michiko,
Kawashima Yumiko,
Shimamoto Mitoshi
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb02053.x
Subject(s) - immunoassay , antibody , chemiluminescence , immunofluorescence , microbiology and biotechnology , antigen , chemistry , immunoglobulin g , agglutination (biology) , chromatography , virology , biology , immunology
A fully automated chemiluminescence immunoassay was developed for the detection of antibodies to HTLV‐I. We used partially purified viral antigens coated on small polystyrene beads together with acridinium ester‐labeled anti‐human immunoglobulin G mouse immunoglobulin G in this method. A good agreement was observed between our proposed method, the indirect immunofluorescence assay, the particle‐agglutination test and the enzyme immunoassay. This new method, which is simple, sensitive, specific and rapid, should be useful for mass screening of anti‐HTLV‐I antibodies.