Open AccessCarboxyl‐terminal Heparin‐binding Fragments of Platelet Factor 4 Retain the Blocking Effect on the Receptor Binding of Basic Fibroblast Growth Factor
Author(s) -
Sato Yasufumi,
Waki Michinori,
Ohno Motonori,
Kuwano Michihiko,
Sakata Toshiie
Publication year - 1993
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1993.tb00163.x
Subject(s) - basic fibroblast growth factor , receptor , fibroblast growth factor receptor , binding site , growth factor , fibroblast growth factor , fibroblast growth factor receptor 2 , heparin , chemistry , fibroblast growth factor receptor 3 , endogeny , platelet , growth factor receptor , platelet derived growth factor , biochemistry , fibroblast growth factor receptor 1 , microbiology and biotechnology , biology , platelet derived growth factor receptor , immunology
Platelet factor 4 (PF‐4) blocks the binding of basic fibroblast growth factor (bFGF) to its receptor. In the present study, we constructed carboxyl‐terminal fragments, which represent the heparin‐binding region of the PF‐4 molecule, and examined whether these synthetic peptides retain the blocking effects on the receptor binding of bFGF. Synthetic peptides inhibited the receptor binding of bFGF. Furthermore, they inhibited the migration and tube formation of bovine capillary endothelial cells in culture (these phenomena are dependent on endogenous bFGF).