Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent  in situ  Hybridization | Zendy

Inazawa Johji | Zendy; Ariyama Takeshi | Zendy; Abe Tatsuo | Zendy

Open Access

Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization

Author(s) -

Inazawa Johji,

Ariyama Takeshi,

Abe Tatsuo

Publication year - 1992

Publication title -

japanese journal of cancer research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.035

H-Index - 141

eISSN - 1349-7006

pISSN - 0910-5050

DOI - 10.1111/j.1349-7006.1992.tb02753.x

Subject(s) - metaphase , biology , microbiology and biotechnology , fluorescence in situ hybridization , tumor suppressor gene , rhodamine , fluorescein isothiocyanate , in situ hybridization , hybridization probe , fluorescence , dna , gene , genetics , chromosome , gene expression , optics , carcinogenesis , physics

Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi‐color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three‐color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo‐color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23–p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors.

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