Open AccessOligonucleotide Sequences Required for Natural Killer Cell Activation
Author(s) -
Kuramoto Etsuro,
Yano Osamu,
Kimura Yoshimitsu,
Baba Makoto,
Makino Tadashi,
Yamamoto Saburo,
Yamamoto Toshiko,
Kataoka Tetsuro,
Tokunaga Tohru
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb02734.x
Subject(s) - palindrome , palindromic sequence , oligonucleotide , biology , dna , sequence (biology) , microbiology and biotechnology , genetics , computational biology , gene , crispr
Based on the previous finding that certain 30‐mer single‐stranded oligodeoxyribonucleotides (oligonucleotides) having particular 6‐mer palindromic sequences could induce interferon‐alpha and ‐gamma, and enhance natural killer activity, the present study was carried out to clarify the entire relationship between the activity and the sequence of 30‐mer oligonucleotides. The results indicated that the activity depended critically on the presence of particular palindromic sequences including the 5 ‐CG‐3 motif(s). The size and the number of palindromes as well as the extra‐palindromic sequences also influenced the activity. An oligonucleotide with a 10‐mer palindrome and extra‐palindromic oligoguanylate sequences showed the strongest activity among the oligonucleotides tested.