Open AccessUse of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
Author(s) -
Yagyu Toshio,
Monden Takushi,
Tamaki Yasuhiro,
Morimoto Hideki,
Takeda Tsutomu,
Kobayashi Tetsuro,
Shimano Takashi,
Murakami Hiroki,
Mori Takesada
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb02346.x
Subject(s) - immunotherapy , monoclonal antibody , lymph node , medicine , lymph , antibody , colonic carcinoma , immunology , cancer , cancer immunotherapy , monoclonal , immune system , cancer research , pathology , carcinoma
Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the host's immunity.