Open AccessLow Frequency of Rearrangements of the ret and trk Proto‐oncogenes in Japanese Thyroid Papillary Carcinomas
Author(s) -
Wajjwalku Worawidh,
Nakamura Shigeo,
Hasegawa Yasuhisa,
Miyazaki Koichi,
Satoh Yasuyuki,
Funahashi Hiroomi,
Matsuyama Mutsushi,
Takahashi Masahide
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb01963.x
Subject(s) - trk receptor , thyroid carcinoma , thyroid , gene rearrangement , adenoma , proto oncogene proteins c ret , pathology , cancer research , biology , medicine , endocrinology , receptor , gene , genetics , neurotrophin , neurotrophic factors , glial cell line derived neurotrophic factor
We investigated the frequency of rearrangements of the ret and trk proto‐oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto‐oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase‐polymerase chain reaction method showed that the ret rearrangement‐positive tumor contained the PTC/ref TPC chimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto‐oncogene was expressed at high levels in one of two trk rearrangement‐positive tumors. Our results indicated that the frequency of rearrangements of these proto‐oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.