Open AccessMechanism of Synergistic Cytotoxic Effect between Tumor Necrosis Factor and Hyperthermia
Author(s) -
Yamauchi Naofumi,
Watanabe Naoki,
Maeda Masahiro,
Okamoto Tetsuro,
Sasaki Hiroyoshi,
Tsuji Naoki,
Tsuji Yasushi,
Umeno Hiroshi,
Akiyama Shinichiro,
Niitsu Yoshiro
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb01962.x
Subject(s) - hyperthermia , cytotoxic t cell , chemistry , reactive oxygen species , tumor necrosis factor alpha , in vivo , necrosis , hydroxyl radical , biochemistry , enzyme , dimethyl sulfoxide , in vitro , biology , immunology , medicine , radical , microbiology and biotechnology , organic chemistry
We previously reported that recombinant human tumor necrosis factor (rhTNF) and hyperthermia had a synergistic effect against tumors, in vitro and in vivo. We have now investigated the mechanism of this synergy by measuring the lysosomal enzyme activity and hydroxyl radical production of L‐M cells treated with rhTNF and/or hyperthermia. A synergistic activation of lysosomal enzyme and the induction of hydroxyl radical production in L‐M cells treated with both rhTNF and hyperthermia was observed. A synergistic cytotoxic effect was observed when rhTNF and hyperthermia were combined, and was inhibited by the addition of a reactive oxygen scavenger, dimethyl sulfoxide or bipyridine. The results show that the augmenting effect of hyperthermia on lysosomal enzyme activation and induction of hydroxyl radical production by rhTNF plays an important role in the synergistic cytotoxic effect.