Open AccessThe Number of Platinum Atoms Binding to DNA, RNA and Protein Molecules of HeLa Cells Treated with Cisplatin at Its Mean Lethal Concentration
Author(s) -
Akaboshi Mitsuhiko,
Kawai Kenichi,
Maki Hirotoshi,
Akuta Keizo,
Ujeno Yowri,
Miyahara Tokiharu
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb01959.x
Subject(s) - hela , cisplatin , dna , platinum , rna , molecule , chemistry , microbiology and biotechnology , cancer research , biology , in vitro , biochemistry , chemotherapy , genetics , gene , organic chemistry , catalysis
HeLa S‐3 cells were treated with 195m Pt‐radiolabeled cis ‐diamminedichloroplatinum(II) (CDDP) under various conditions, and the relationship between lethal effect and the number of Pt atoms binding to DNA, RNA and proteins was examined. The mean lethal concentrations for the cells treated with CDDP at 37°C for 1, 2 and 3 h were 2.8, 2.0 and 1.1 μg/ml, respectively. By using identically treated cells, the number of Pt atoms combined with DNA, RNA and protein molecules were determined after fractionation of the cells using the method of Schneider. In this way, the Do values given as the drug concentration were substituted for the number of Pt atoms combined with each fraction, then the target volumes expressed as the reciprocals of Do values were calculated for each fraction. The results provide strong support for the idea that DNA is the primary target for cell killing by CDDP, and the target volumes were 5.17 × 10 4 , 5.71 × 10 4 and 1.03 × 10 4 nucleotides for 1, 2 and 3 h treated cells, respectively.