Open AccessPhenotypic and Genotypic Lineage Switch of a Lymphoma with Shared Chromosome Translocation and T‐Cell Receptor γ Gene Rearrangement
Author(s) -
Yamamoto Kazuhito,
Osada Hirotaka,
Seto Masao,
Ogura Michinori,
Suzuki Hisamitsu,
Utsumi Kazuhiko R.,
Oyama Atsushi,
Ariyoshi Yutaka,
Nakamura Shigeo,
Kurita Souji,
Takahashi Toshitada,
Ueda Ryuzo
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb01951.x
Subject(s) - chromosomal translocation , biology , gene rearrangement , lymphoma , t cell receptor , clone (java method) , karyotype , t cell , cancer research , t cell lymphoma , microbiology and biotechnology , chromosome , genetics , gene , immunology , immune system
A case of non‐Hodgkin's lymphoma showed a phenotypic and genotypic cell lineage switch twice during nine years of his clinical history; first, T‐cell type, pleomorphic small cell lymphoma developed, followed by B‐cell type, diffuse centroblastic/centrocytic lymphoma, and finally T‐zone lymphoma without follicles again developed, from which AST‐1 cultured cell line was established. Karyotype analysis demonstrated a shared abnormal chromosome, der(1)t(1;?)(p36;?), among the first relapsed B‐cell tumor, the second relapsed T‐cell tumor and AST‐1 cell line. Furthermore, T‐cell receptor (TCR) γ gene rearrangement bands of the same size were observed in the first relapsed B‐cell tumor and the second relapsed T‐cell tumor as well as AST‐1 cell line. These results suggested that both relapsed tumors of different cell lineages are derived from a common malignant clone, presumably a committed lymphoid stem cell. A unique translocation, t(2;14)(q37;q11.2), which may involve TCR δ/α gene complex, was observed in the second relapsed tumor and AST‐1 cells. To attempt to isolate the breakpoint of this translocation, the configuration of TCR δ/α gene complex was studied. The result showed that two rearrangements of TCR α gene detected with J α probes were the products of the normal TCR rearrangement process, and were not involved in the translocation at this region. This patient, together with the AST‐1 cell line, provided us a unique opportunity to study the development and clonal evolution of malignant lymphoma.