Open AccessSoluble Interleukin‐6 Receptor Is Released from Receptor‐bearing Cell Lines in vitro
Author(s) -
Nakajima Toshiharu,
Yamamoto Shinji,
Cheng Ming,
Yasukawa Kiyoshi,
Hirano Toshio,
Kishimoto Tadamitsu,
Tokunaga Tohru,
Honda Mitsuo
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb00117.x
Subject(s) - cell culture , receptor , microbiology and biotechnology , biology , interleukin 6 receptor , in vitro , cell surface receptor , antibody , chemistry , cytokine , interleukin , immunology , biochemistry , genetics
Soluble interleukin‐6 receptor (sIL‐6R) was found to be spontaneously released from human myeloma cell line U266 cells into culture supernatant, and was quantitatively measured with a fluorescence sandwich enzyme‐linked immunosorbent assay employing antibodies specific to IL‐6R. The supernatant IL‐6R was generated only from IL‐6R‐positive cell lines; myeloma cell lines RPMI8226 and RPMI1788, and myelomonocytic cell lines U937, THP‐1, and HL‐60. In contrast, it was not released from the IL‐6R‐negative cells; T cell line MoIt‐4 and Burkitt lympboma cell line Raji. SDS‐PAGE analysis of the soluble IL‐6R from U266 cells suggested a molecular weight of approximately 50‐55 kDa, 25–30 kDa smaller than the mature cell surface receptor. These results suggest that the generation of soluble IL‐6R may be a maker of myeloma cells and myelomonocytic cells.