Open AccessUbiquitous Presence in Mammalian Cells of Enzymatic Activity Specifically Cleaving 8‐Hydroxyguanine‐containing DNA
Author(s) -
Yamamoto Fuyumi,
Kasai Hiroshi,
Bessho Tadayoshi,
Chung MyungHee,
Inoue Hideo,
Ohtsuka Eiko,
Hori Tomokatsu,
Nishimura Susumu
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb00114.x
Subject(s) - enzyme , dna , biochemistry , gel electrophoresis , cleavage (geology) , chemistry , polyacrylamide gel electrophoresis , residue (chemistry) , microbiology and biotechnology , polyacrylamide , biology , paleontology , fracture (geology)
Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8‐hydroxyguanine (oh 8 Gua) residues in various mammalian cells. To detect this activity, we used a synthetic double‐stranded DNA containing a single oh 8 Gua at a defined position as the substrate, and analyzed the products of enzymatic digestion by polyacrylamide gel electrophoresis. Two cleavage sites near the oh 8 Gua residue were detected with partially purified fractions from cow brain and rat liver, and also with preparations from all mammalian tissues examined. These results suggest that enzymatic activity for the removal of oh 8 Gua from DNA is widely distributed in mammalian cells.