Open AccessShort Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
Author(s) -
Iwamoto Aikichi,
Yoshikawa Hiroyuki,
Kitagawa Kyoko,
Igarashi Hiroko,
Kawana Takashi,
Yoshikura Hiroshi
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb00107.x
Subject(s) - primer (cosmetics) , polymerase chain reaction , nucleic acid sequence , biology , sequence (biology) , microbiology and biotechnology , nucleotide , genetics , dna sequencing , virology , computational biology , dna , gene , chemistry , organic chemistry
A consensus primer‐mediated polymerase chain reaction devised to amplify a short sequence in LI region (L1‐PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1‐PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross‐hybridization under the stringent condition.