Open AccessPrimer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
Author(s) -
Sugawara Isamu,
Watanabe Masatoshi,
Masunaga Atsuko,
Itoyama Shinji,
Ueda Kazumitsu
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb00076.x
Subject(s) - primer (cosmetics) , polymerase chain reaction , microbiology and biotechnology , reverse transcriptase , messenger rna , biology , reverse transcription polymerase chain reaction , inverse polymerase chain reaction , primer dimer , real time polymerase chain reaction , genetics , gene , chemistry , nested polymerase chain reaction , multiplex polymerase chain reaction , organic chemistry
We have developed a sensitive reverse transcription (RT)‐polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr ‐specific primer sets, mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should he used for effective RT‐PCR.