Open AccessTransforming Activity of a Synthetic c‐Ha‐ras Gene Containing O 6 ‐Methylguanine in Codon 12
Author(s) -
Kamiya Hiroyuki,
Miura Kazunobu,
Ohtomo Noriko,
Nishimura Susumu,
Ohtsuka Eiko
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01934.x
Subject(s) - transfection , gene , mutagenesis , microbiology and biotechnology , dna , adduct , chemistry , mutation , gene conversion , biology , gene mutation , biochemistry , recombination , organic chemistry
A mutagenic DNA‐adduct, O 6 ‐methylguanine, was introduced into codon 12 of the synthetic c‐Ha‐ ras gene by cassette mutagenesis. Transfection of this modified ras gene into normal NIH3T3 cells by the calcium phosphate procedure resulted in significant induction of focus formation. The ras gene inserted into the transformed cells was found to have a G to A transition at the position of the modified base. These results indicate that an O 6 ‐methylguanine residue in DNA may lead to a mutation and be one cause of activation of the ras gene.