Open AccessInterleukin‐2‐inducible Killer Activity and Its Regulation by Blood Monocytes from Autologous Lymphocytes of Lung Cancer Patients
Author(s) -
Sone Saburo,
Kunishige Eiji,
Fawzy Farid,
Yanagawa Hiroaki,
Nil Akihiko,
Maeda Kenichi,
Atagi Shinji,
Heike Yuji,
Nishioka Yasuhiko,
Mizuno Kazuhito,
Ogura Takeshi
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01908.x
Subject(s) - lymphokine activated killer cell , lung cancer , medicine , peripheral blood mononuclear cell , immunology , adenocarcinoma , lung , interleukin 2 , monocyte , cancer , percoll , in vitro , cancer research , cytokine , pathology , immune system , biology , t cell , interleukin 21 , biochemistry
The ability of blood lymphocytes of newly diagnosed lung cancer patients to respond to interleukin 2 (IL‐2) to become IL‐2‐activated killer (LAK) cells and its regulation by autologous monocytes were examined. LAK activity was measured by 31 Cr release assay. The abilities of lymphocytes among blood mononuclear cells (MNC) of subjects of different ages without malignancies to generate LAK activity against NK‐cell resistant Daudi cells and lung adenocarcinoma (PC‐9) cells were very similar. The LAK activity of blood MNC of lung cancer patients was also nearly the same as that of blood MNC of control subjects. There was no significant difference in IL‐2‐inducible LAK activity between MNC of patients with small cell lung cancer (SCLC) and those of patients with non‐SCLC. Monocytes and lymphocytes were separated from blood MNC on a one‐step Percoll gradient. Monocytes of lung cancer patients were found to augment in vitro induction of LAK activity by IL‐2 of autologous blood lymphocytes. In contrast, endotoxin‐stimulated monocytes suppressed LAK induction of autologous lymphocytes of cancer patients. These findings suggest that administration of IL‐2 and LAK cells induced in vitro may be of benefit in the treatment of lung cancer.