Open AccessEpigenetic Control of Tumor Cell Morphology
Author(s) -
Horio Keizo,
Yoshikura Hiroshi,
Kawabata Masahiro,
Odawara Takashi,
Sudo Katsuko,
Fujitani Yohei,
Lee Ganghong,
Iwamoto Aikichi
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01903.x
Subject(s) - rous sarcoma virus , clone (java method) , biology , cell culture , cell type , microbiology and biotechnology , cell , virus , virology , gene , genetics
XC cell line derived from a single rat cell transformed by the Prague strain of Rons sarcoma virus produced morphologically different colonies. Among them, two distinct cell types consisting of thick, fusiform cells (L‐type), and of flat, polygonal cells (R‐type) were apparent. By repeated subclonings, pure cultures, L1 and R1, respectively, were obtained. These clones underwent morphological conversion during prolonged culture; L‐type colonies appeared in the R‐type clone and vice versa. The kinetic curve suggested that the conversion was multi‐stepped. When inoculated into nude mice, L‐type cells produced much larger tumors at a higher frequency than R‐type cells, and the tumors induced by these two clones were histologically different. The expression of v‐ src gene was higher in L‐type than in R‐type cells at both mRNA and protein levels.