Open AccessTime‐ and Dose‐dependent Induction of Invasive Urinary Bladder Cancers by N‐Ethyl‐N‐(4‐hydroxybutyl)nitrosamine in B6C3F 1 Mice
Author(s) -
Tamano Seiko,
Hagiwara Akihiro,
Suzuki Emako,
Okada Masashi,
Shirai Tomoyuki,
Fukushima Shoji
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01900.x
Subject(s) - nitrosamine , urinary system , urinary bladder , carcinogen , urology , pathology , medicine , cancer research , chemistry , biology , biochemistry
A sequential investigation of N‐ethyl‐N‐(4‐hydroxybutyl)nitrosamine (EHBN) bladder carcino‐genesis was performed in male B6C3F 1 mice maintained ad libitum on tap water containing 0.025% EHBN for 4, 12, 20, 28 and 36 weeks. A total of 81 invasive tumors, comprising 55 smiamous cell carcinomas (SCCs) (68%), 25 transitional cell carcinomas (TCCs) (31%) and 1 adenocarcinoma (1%) were found. Of these, 23 (22 SCCs and 1 TCC) demonstrated invasion to the prostate, 3 metastasized to the lung, and 2 spread by peritoneal seeding. The anaplastic grade and extent of invasion of the SCCs significantly exceeded those of the TCCs. The results suggested a histogenetic pathway from simple dysplasia through papillary or nodular dysplasia and/or carcinoma in situ to eventual development of invasive carcinomas