Open AccessMultipotent and Committed CD34 + Cells in Bone Marrow Transplantation
Author(s) -
Ema Hideo,
Suda Toshio,
Nakauchi Hiromitsu,
Nakamura Yukio,
Iwama Atsushi,
Imagawa Shigehiko,
Akutsu Miyuki,
Kano Yasuhiko,
Kato Shunichi,
Yabe Miharu,
Yoshida Minoru,
Sakamoto Shinobu,
Amemiya Youichi,
Miura Yasusada
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01885.x
Subject(s) - cd34 , bone marrow , haematopoiesis , stem cell , progenitor cell , biology , multipotent stem cell , immunology , transplantation , microbiology and biotechnology , medicine
In order to study the role of CD34 + cells in hematological recovery following bone marrow transplantation (BMT), bone marrow cells stained with HPCA‐1 (CD34) and MY‐9 (CD33) monoclonal antibodies were analyzed by using a fluorescence‐activated cell sorter on or about days 14 and 28, as well as at later times, following BMT in 6 recipients. Single cell cultures of CD34 + cells were also performed to evaluate their in vitro hematopoietic function. CD34 + cells were detectable in bone marrow cells on day 14. More than 80% of CD34 + cells co‐expressed the CD33 antigen, and macrophage (Mac) colony‐forming cells predominated among total colony‐forming cells of CD34 + cells. In normal bone marrow cells, CD34 + , CD33 + cells amounted to about 40% of CD34 + cells, and the incidences of erythroid bursts, granulocyte/macrophage (GM) colonies, and Mac colonies were similar to each other. After more than 10 weeks, CD34 + , CD33 − cells gradually recovered, as erythroid burst colony‐forming cells increased following GM colony‐forming cells. This phenomenon was well‐correlated with the time course of peripheral blood cell recovery. CD34 + , CD33 + cells as committed progenitors and CD34 + , CD33 − cells as multipotent stem cells have distinctive biological behaviors in BMT.