Open AccessInhibitory Effect of 2‐0‐Octadecylascorbic Acid in Agglutination Assay with Concanavalin A; Short‐term Examination of Rat Urinary Bladder Carcinogenesis
Author(s) -
Suzuki Mika,
Wakabayashi Keiji,
Sone Hideko,
Kushida Hiromi,
Sugiyama Kiyoshi,
Kakizoe Tadao,
Nagao Minako,
Sugimura Takashi
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01860.x
Subject(s) - concanavalin a , urinary bladder , medicine , carcinogenesis , chemistry , ascorbic acid , endocrinology , pathology , biochemistry , cancer , in vitro , food science
A derivative of ascorbic acid, 2‐ O ‐octadecylascorbic acid (CV‐3611), is a strong scavenger of active oxygen species. We examined the effect of CV‐3611 on a short‐term test of bladder Carcinogenesis, using Concanavalin A (Con A) ‐dependent agglutination of isolated bladder epithelial cells. Rats were given 0.01% N ‐butyl‐ N (4‐hydroxybutyl)nitrosamine (BHBN) for 1 week, and then 5% sodium saccharin or 2% DL‐tryptophan or 0.01% BHBN alone or with 0.002, 0.006 or 0.02% CV‐3611 for 3 weeks. Treatment with CV‐3611 reduced the effects of the bladder tumor promoters sodium saccharin and DL‐tryptophan by 48–86 and 65–87%, respectively. CV‐3611 also reduced the number of aggregates of bladder epithelial cells from rats treated with BHBN for 4 weeks. These results suggest that CV‐3611 has a suppressive effect on rat bladder Carcinogenesis.