Open AccessOverexpression of c‐Myc Inhibits the Appearance of a Specific DNase I Hypersensitive Site in the β‐Globin Chromatin in Murine Erythroleukemia Cells
Author(s) -
Takada Shinji,
Obinata Masuo
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01858.x
Subject(s) - hypersensitive site , chromatin , dnase i hypersensitive site , microbiology and biotechnology , globin , deoxyribonuclease i , biology , gene , locus control region , gene expression , promoter , genetics , base sequence
To investigate the molecular mechanism of regulation of differentiation by c‐Myc, we examined the acquisition of DNase I hypersensitivity by; β ‐globin chromatin in an MEL cell transformant (38–2) in which the c ‐myc gene was placed under the control of a metallothionein gene promoter. Of the three DNase I hypersensitive sites around the β ‐globin gene which were detected in MEL cells after DMSO treatment, the appearance of a specific DNase I hypersensitive site near the promoter was greatly reduced by the Overexpression of c‐Myc. This result suggests that c‐Myc regulates β ‐globin expression by interfering with the establishment of an active chromatin conformation around the β ‐globin gene.