Open AccessChromosomal Insertion and Amplification of Human Papillomavirus 16 DNA Sequences in a Cell Line of Argyrophil Small Cell Carcinoma of the Uterine Cervix
Author(s) -
Hori Tadaaki,
Ichimura Hiroshi,
Minamihisamatsu Masako,
Takahashi Eiichi,
Yamauchi Masatake,
Hama Yuko,
Kurimura Osamu,
Yamasaki Masato,
Kurimura Takashi
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01857.x
Subject(s) - biology , fluorescence in situ hybridization , dna , interphase , microbiology and biotechnology , papillomaviridae , cell culture , carcinoma , gene duplication , nucleic acid thermodynamics , genetics , gene , cancer , chromosome , cervical cancer , base sequence
The chromosomal location of human papillomavirus (HPV) 16 DNA sequences integrated in a cell line derived from argyrophil small cell carcinoma of the uterine cervix was determined by means of fluorescence in situ hybridization (FISH). The HPV 16 DNA sequences were integrated near a fragile site and the location of the c‐myc oncogene at 8q24.1. Amplification of the integrated viral sequences resulted in an abnormally banded region. The amplified HPV 16 DNA sequences were also detected in every interphase nucleus by FISH.