Open AccessHuman Monoclonal Antibody Detects a Cell Surface Antigen Expressed on Hematopoietic Malignant Cells of Lymphoid Lineage
Author(s) -
Iizasa Toshihiko,
Yamaguchi Yutaka,
Tagawa Masatoshi,
Fujisawa Takehiko,
Saito Hiroaki,
Kondo Haruki,
Matsuo Yoshinobu,
Minowada Jun,
Taniguchi Masaru
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01831.x
Subject(s) - antigen , chronic myelogenous leukemia , monoclonal antibody , haematopoiesis , biology , leukemia , myeloid , antibody , peripheral blood mononuclear cell , lymphoma , immunology , microbiology and biotechnology , cancer research , stem cell , in vitro , biochemistry , genetics
An antigen with a molecular weight of 150 kilodaltons expressed on certain leukemia and lymphoma cells was recognized by a human monoclonal antibody (3H12), which had been established by the fusion of lymphocytes from a small cell lung cancer patient with a mouse myeloma cell line (P3U1). Peripheral blood mononuclear cells from 3 out of 4 cases with lytnphoid crisis of chronic myelogenous leukemia (CML) were positively stained by 3H12, while cells from 5 cases with myeloid crisis of CML did not react to this antibody. The antibody did not show any reactivity to cells from the chronic phase of CML, other types of leukemias or normal hematopoietic cells. We further examined 29 cell lines of hematopoietic origin and found that 2 undifferentiated cells (BV‐173 and K‐562) reacted to the 3H12 antibody. In addition, we found that 3 out of 6 Burkitt lymphoma cells (DAUDI, RAJI and HR1K) reacted to 3H12. Taken together, these results suggest that the antigen recognized by 3H12 is a differentiation‐associated antigen expressed on immature lymphoid cells, and could potentially be a reliable cell lineage marker.