Open AccessRelease of Esterase from Murine Lymphokine‐activated Killer Cells in Antibody‐dependent Cellular Cytotoxic Reaction
Author(s) -
Kato Kazunori,
Agatsuma Toshinori,
Tanabe Toshifumi,
Masuko Takashi,
Hashimoto Yoshiyuki
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01830.x
Subject(s) - lymphokine activated killer cell , antibody dependent cell mediated cytotoxicity , cytotoxic t cell , lymphokine , monoclonal antibody , chemistry , biology , microbiology and biotechnology , antibody , immunology , immune system , biochemistry , in vitro , interleukin 21
Release of granule enzyme(s) (BLT esterase) in the antibody dependent lymphokine‐activated killer (LAK) cell‐mediated cytotoxic reaction (LAK ADCC) was studied using LAK cells induced from murine splenocytes and thymocytes, various human tumor cells and relevant monoclonal antibodies (mAbs) to the tumor cells. BLT esterase was not significantly released from LAK cells in direct LAK cell‐mediated cytotoxic reactions (LAK CMC). However, cultures of LAK cells and IgG‐coated target tumor cells resulted in release of the enzyme concomitantly with target cell lysis, although esterase release proceeded faster than target cell lysis. Anti‐LFA‐1 mAb showed an inhibitory effect on LAK CMC but not on either LAK ADCC or BLT esterase release in the ADCC. These results indicate that exocytosis of granule enzyme from LAK cells is triggered by stimulation of Fc receptor on LAK cells and that LAK CMC and LAK ADCC differ in their lytic mechanism in terms of the release of BLT esterase.