Open AccessPlatelet Aggregation Induced by Adenosine Diphosphate Released from Cloned Murine Fibrosarcoma Cells Is Positively Correlated with the Experimental Metastatic Potential of the Cells
Author(s) -
Mogi Yoshihiro,
Kogawa Katsuhisa,
Takayama Tetsuji,
Yoshizaki Naohito,
Bannai Kiyoshi,
Muramatsu Hirohito,
Koike Kazuhiko,
Kohgo Yutaka,
Watanabe Naoki,
Niitsu Yoshiro
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01828.x
Subject(s) - clone (java method) , adenosine diphosphate , apyrase , platelet , fibrosarcoma , chemistry , cell culture , biology , biochemistry , microbiology and biotechnology , cancer research , endocrinology , platelet aggregation , immunology , gene , genetics
We established five clones (ML‐01, ML‐02, MH‐01, MH‐02, MH‐03) from murine 3‐methylcholan‐threne‐induced fibrosarcoma A (Meth A), and investigated their experimental metastatic potentials in relation to their platelet‐aggregating activities. A clone with a high metastatic potential (MH‐02) showed a characteristic biphasic pattern of platelet aggregation, of which the first peak was not present in the aggregation patterns of the clone with low metastatic potential (ML‐01). The first peak was eliminated by treatment of the cells with apyrase, indicating that adenosine diphosphate (ADD was the causative substance of this particular peak. The metastatic potential of clones correlated well with the ADP concentration of the culture media. These results suggest that the increased ADP production and consequential enhancement of platelet‐aggregating activity are closely related to the increment of pulmonary metastatic potential of MH‐02 clone.