Open AccessRelation of 8‐Hydroxydeoxyguanosine Formation in Rat Kidney to Lipid Peroxidation, Glutathione Level and Relative Organ Weight after a Single Administration of Potassium Bromate
Author(s) -
Sai Kimie,
Takagi Atsuya,
Umemura Takashi,
Hasegawa Ryuichi,
Kurokawa Yuji
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01824.x
Subject(s) - lipid peroxidation , potassium bromate , chemistry , glutathione , endocrinology , medicine , kidney , antioxidant , biochemistry , biology , enzyme , catalysis
Changes in kidney levels of 8‐hydroxydeoxyguanosine (8‐OH‐dG), lipid peroxidation (LPO), glutathione (GSH) and relative organ weight were examined 6, 24, 48, 72 and 96 h after a single i.p. administration of potassium bromate (KBrO 3 ) at a dose of 70 mg/kg to male F344 rats. The 8‐OH‐dG level was significantly increased 24 h after the treatment at this dose and thereafter gradually decreased. On the other hand, significant elevation in LPO level was observed from 6 h after the treatment with a continuous increase up to a plateau at 48 h and no subsequent drop. GSH level was significantly raised from 6 to 72 h, and relative kidney weight varied in almost the same manner as the 8‐OH‐dG level. Investigation of the dose‐response relation revealed the 8‐OH‐dG and LPO levels to be significantly increased from a dose of 40 mg/kg KBrO 3 in a dose‐dependent manner. The results suggest that enhanced formation of 8‐OH‐dG in kidney DNA due to KBrO 3 is closely related to the increase in LPO levels.