Open AccessEstablishment and Characterization of an Epithelial Cell Line with Quasi‐normal Chromosomes from a Tubular Adenoma of a Familial Polyposis Coli Patient
Author(s) -
Kawaguchi Tokuichi,
Miyaki Michiko,
Masui Tohru,
Watanabe Motoko,
Ohta Hirotoshi,
Maruyama Masakazu,
Utakoji Tadashi,
Kitagawa Tomoyuki
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01820.x
Subject(s) - adenoma , biology , carcinogenesis , karyotype , cell culture , gene , mutation , cancer research , point mutation , chromosome , epithelium , gene duplication , cell division , cell , microbiology and biotechnology , pathology , genetics , medicine
An epithelial cell line designated FPCK‐1 has been established from a tubular adenoma developing in a male familial polyposis coli (FPC) patient. The FPCK‐1 cells grow very slowly with adundant mucus production and have been maintained stably for 3 years in culture. No growth was evident either in soft agar or nude mice, FPCK‐1 cells present a normal male karyotype and do not show loss of specific loci on chromosomes 5,17,18, and 22 which have been reported to be lost frequently in human colon carcinomas. The cells have neither a point mutation on codon 12 of K‐ ras gene nor gene amplification of myc , c‐H‐ ras , and/or c‐K‐ ras genes. These results thus suggest the existence of hitherto unknown causative event(s) underlying adenoma development in FPC patients. The FPCK‐1 cell line should prove useful for further analytical investigation of the multiple steps involved in human colon carcinogenesis.