Open AccessEffects of the Tumor Inhibitor IKP‐104, a 4(l H )‐Pyridinone Derivative, on Cytoskeletal Microtuhules of Cultured Tumor Cells
Author(s) -
Mizuhashi Fukutaro,
Murata Kyoji,
Kitagaki Tadaharu,
Isao Isao
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01818.x
Subject(s) - cytoskeleton , tubulin , mitosis , microtubule , multinucleate , metaphase , microbiology and biotechnology , cytoplasm , interphase , vinblastine , biology , microtubule organizing center , cell cycle , pathology , chemistry , cell , biochemistry , genetics , medicine , gene , centrosome , chromosome , chemotherapy
The effects of IKP‐104, a 4(l H )‐pyridinone derivative, on the mitotic profile and cytoskeletal microtubule dynamics of cultured B16 melanoma cells were examined in order to investigate the mechanism of its antitumor activity. The exposure to IKP‐104 caused accumulation of cells in abnormal metaphase with chromosomes scattered within the cytoplasm and induced polyploid and multinucleate cells as detected by differential staining microscopy with brilliant blue R and safranin O. An immunofluorcsceiicc study with monoclonal anti‐α‐tubulin antibody revealed that IKP‐104 diminished cytoskeletal microtubules of both interphase and mitotic cells, resulting in induction of a few fragments resembling “microtubular bundles” induced by vinblastine (VLB). These results indicated that IKP‐104 arrests cells in the mitotic phase by inhibition of polymerization and induction of depolymerization of cytoskeletal microtubules, similarly to VLB.