Open AccessDetection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
Author(s) -
Hashido Madoka,
Kanda Tadahito,
Zanma Soichi,
Watanabe Sumie,
Komiyama Naoki,
Yoshikawa Hiroyuki,
Yamaguchi Nobuo,
Kawana Takashi,
Kunito Kunito
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01813.x
Subject(s) - antibody , immunofluorescence , human papillomavirus , biology , virology , fusion protein , microbiology and biotechnology , immunology , medicine , gene , recombinant dna , biochemistry
We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme‐linked immunosorbent assay with bacterially expressed fusion protein lac‐ E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS‐1 cells. The anti‐E7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted diseases, and healthy adults. The data indicate that expression of the E7 protein is closely related to cervical carcinoma.