Open AccessHuman Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
Author(s) -
Itoh Yoshinori,
Kohgo Yutaka,
Watanabe Naoki,
Kanisawa Yuji,
Sakamaki Sumio,
Takahashi Minoru,
Hirayama Yasuo,
Ono Hiroyuki,
Himeno Takeshi,
Niitsu Yoshiro
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01781.x
Subject(s) - tumor necrosis factor alpha , transfection , cytotoxicity , cancer research , cell culture , tumor infiltrating lymphocytes , k562 cells , genetic enhancement , biology , necrosis , immunology , cytokine , cd30 , pathology , microbiology and biotechnology , in vitro , immunotherapy , medicine , tumor cells , gene , immune system , biochemistry , genetics
Human tumor–infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) a gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatant and exhibited cytotoxicity against established cell lines, such as K562 and Daudi, and autologous tumor cells. The TNF gene–transfected TILs exhibited an augmented killing of autologous tumor cells.