Open AccessEffects of Methylthiodeoxyadenosine and Its Analogs on in vitro Invasion of Rat Ascites Hepatoma Cells and Methylation of Their Phospholipids
Author(s) -
Kido Junichi,
Ashida Yoshiyuki,
Shinkai Kiyoko,
Akedo Hitoshi,
Isoai Atsushi,
Kumagai Hiromichi,
Hideo Hideo
Publication year - 1991
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1991.tb01764.x
Subject(s) - in vitro , phospholipid , biology , phosphatidylethanolamine , biochemistry , membrane fluidity , cell culture , microbiology and biotechnology , methylation , membrane , dna , phosphatidylcholine , genetics
The relationship between tumor invasiveness in vitro and methyiation of plasma membrane phospholipids was investigated. For this purpose, two hepatoma cell lines, Cl‐30 and LC‐AH, were used which show specific penetration to below cultured monolayers of mesothelial cells from rat mesentery and endothelial cells from calf pulmonary artery, respectively. Methylthiodeoxyadenosine (MTA) and five of its analogs, difluoro‐MTA, deoxyadenosine, sinefungin, phenylthiodeoxyadenosine and fluorophenylthiodeoxyadenosine, inhibited the invasion of the tumor cells without affecting their proliferation. This inhibition was associated with reduction in the incorporation of radioactivity of [methyl‐3H]methionine into cellular phosphatidylethanolamine derivatives without changes in the labelings of RNA and DNA and carboxylmethylation of protein. These compounds also decreased the membrane fluidity of the tumor cells, measured by a steady‐state fluorescence polarization method. Three other MTA analogs (fluorodideoxyadenosine, fluoroazidodideoxyadenosine and flnoroamino‐dideoxyuridine) did not affect the invasiveness of the tumor cells or alter their phospholipid methyiation or membrane fluidity at concentrations that did not inhibit proliferation. These results suggest that the decrease in invasiveness of tumor cells by MTA and its analogs is due to alterations in the phospholipid composition and fluidity of the tumor cell membranes.