Open AccessInduction of Killer Cells from Lymphocytes in Pleural Effusion of Advanced Lung Cancer Patients
Author(s) -
Inoue Yuji,
Shijubo Noriharu,
Uede Toshimitsu
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb03339.x
Subject(s) - cytotoxic t cell , lymphokine activated killer cell , interleukin 2 , lymphokine , medicine , major histocompatibility complex , immunology , lung cancer , monoclonal antibody , cytotoxicity , immunotherapy , cancer research , pleural effusion , pathology , antibody , biology , antigen , immune system , in vitro , cd8 , interleukin 21 , biochemistry
We analyzed the phenotype and cytotoxic ability of pleural exudative lymphocytes (PLEL) which were obtained from 18 advanced lung cancer patients. Freshly isolated PLEL were mainly CD4(+) T cells and had weak natural killer, autologous tumor killing and lymphokine‐activated killer activities. After cultivation of PLEL with interleukin‐2, cytotoxicity of PLEL against autologous tumor cells was increased at 2 weeks, but it was remarkably reduced at 4 weeks. When PLEL were stimulated by mitomycin C‐treated autologous tumor cells during culture, autologous tumor killing activity of PLEL was significantly enhanced even after 4 weeks of cultivation. Cold target inhibition analysis and binding inhibition assays using monoclonal antibodies indicated that autologous tumor stimulation could induce major histocompatibility complex class I restricted cytotoxic T lymphocytes specific for autologous tumor cells in some cases.