Open AccessIdentification of Members of the Protein Phosphatase 1 Gene Family in the Rat and Enhanced Expression of Protein Phosphatase 1α Gene in Rat Hepatocellular Carcinomas
Author(s) -
Sasaki Kazunori,
Shima Hiroshi,
Kitagawa Yoshinori,
Irino Shozo,
Sugimura Takashi,
Nagao Minako
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02690.x
Subject(s) - complementary dna , biology , microbiology and biotechnology , gene , peptide sequence , amino acid , phosphatase , alternative splicing , protein subunit , nucleic acid sequence , gene expression , biochemistry , exon , enzyme
We isolated four kinds of cDNA clones of isotypes of catalytic subunits of protein phosphatase 1 (PP‐1) from rat liver and testis cDNA libraries. For the cloning, cDNA fragments of dis2 ml and dis2 m2, which encode mouse PP‐1 catalytic subunits, were used as probes. Two of the four isotypes were thought to be derived from the same gene and produced by alternative splicing. Based on the comparative study of their nucleotide and deduced amino acid sequences with those reported, these cDNA clones were named rat PP‐1α, PP‐1γ1, PP‐1γ2 and PP‐δ. The deduced amino acid sequences of these four cDNA clones showed about 90% identity. Their amino‐terminal regions were highly conserved, and their differences were mainly in the carboxy‐terminal regions. Furthermore, several amino acids located in the middle regions of the peptides were conserved in all the isotypes of the catalytic subunits of PP‐1, PP‐2A, PP‐2B and PP‐2C. These conserved regions are suggested to be the functional domains of the catalytic subunits of protein phosphatases. Rat hepatocellular carcinomas induced by a food mutagen, 2‐amino‐3,8‐dimethylimidazo[4,5‐ f ]quinoxaline showed increased expression of PP‐1α , but no increased expression of PP‐1γ1, PP‐1γ2 or PP‐1δ . Involvement of PP‐1α in hepatocarcinogenesis or in hepatic cell proliferation was suspected.