Open AccessInhibition of Avian Myeloblastosis Virus Reverse Transcriptase by Aurochloric Acid
Author(s) -
Semba Mari,
Tanaka Noriho,
Yamada Masaatsu,
Takeuchi Fujio,
Matsuta Kunio,
Miyamoto Terumasa,
Hanaoka Fumio,
Ui Michio
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02688.x
Subject(s) - reverse transcriptase , hela , primer (cosmetics) , dna polymerase , microbiology and biotechnology , rna directed dna polymerase , dna polymerase i , dna , polymerase , substrate (aquarium) , enzyme , biology , chemistry , biochemistry , rna , cell , gene , ecology , organic chemistry
We investigated the inhibitory effects of aurochloric acid (AuCl 4 H) on reverse transcriptase (RT) derived from avian myeloblastosis virus and DNA polymerase a (pol. a ) purified from HeLa S3 cells. The activities of RT, pol. a and E. coli DNA polymerase I (pol. I) with dTTP as the substrate were inhibited 50% at AuCl 4 H concentrations of 18 μM , 43 μM and 230 μM , respectively. AuCl 4 H inhibited RT activity competitively with respect to the substrate, dTTP, and uncompetitively with the template/primer, (rA) n (dT) 12‐18 . In assays with dGTP as the substrate, 50% inhibitions of RT, pol. a and pol. I activities were observed at AuCl 4 H concentrations of 100 μM , 450 μM and 580 μM , respectively. AuCl 4 H inhibited RT activity uncompetitively with respect to the substrate, dGTP, and noncompetitively with the template/primer, (rC) n (dG) 12‐18 . AuCl 4 H at concentrations causing more than 50% inhibition of RT activity had little inhibitory effect on the colony‐forming ability of HeLa cells or their syntheses of DNA, RNA and protein.