Open AccessIncrease in the Level of P‐Glycoprotein mRNA Expression in Multidrug‐resistant K562 Cell Lines Treated with Sodium Butyrate Is Not Accompanied with Erythroid Differentiation
Author(s) -
Shibata Hiroyuki,
Kanamaru Ryunosuke,
Sato Toshiaki,
Ishioka Chikashi,
Konishi Yukari,
Ishikawa Akira,
Wakui Akira,
Tsuruo Takashi
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02681.x
Subject(s) - sodium butyrate , k562 cells , p glycoprotein , hemin , northern blot , messenger rna , butyrate , microbiology and biotechnology , cell culture , biology , glycoprotein , mitomycin c , vincristine , chemistry , endocrinology , cell , biochemistry , multiple drug resistance , chemotherapy , enzyme , heme , gene , cyclophosphamide , genetics , fermentation , antibiotics
We examined the effects of hemin, sodium butyrate and mitomycin C on levels of P‐glycoprotein mRNA in human myelogenous K562 cells by northern blot analysis. After treatment with sodium butyrate a dose‐dependent increase of P‐glycoprotein mRNA expression was observed in the adriamycin‐resistant K562 and vincristine‐resistant K562 lines. With 10 m M sodium butyrate, the level of P‐glycoprotein mRNA reached 20 times that of control adriamycin‐resistant K562 and with 30 m M sodium butyrate, it exceeded 5 times that of control vincristine‐resistant K562. In contrast, hemin and mitomycin C had almost no effect on P‐glycoprotein mRNA. In this experiment, since expression of P‐glycoprotein mRNA was not necessarily accompanied with induction of erythroid differentiation, the increased amount of P‐glycoprotein mRNA is unlikely to be a result of differentiation.