Open AccessDetection of the c‐ myc Gene Product in Urinary Bladder Cancer
Author(s) -
Kotake Toshihiko,
Saiki Shigeru,
Kinouchi Toshiaki,
Shiku Hiroshi,
Nakayama Eiichi
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02677.x
Subject(s) - urinary system , bladder cancer , urinary bladder , gene , medicine , urinary bladder cancer , cancer research , gene product , cancer , biology , urology , oncology , gene expression , genetics
Expression of the c‐ myc gene product in urinary bladder cancer was investigated by immunohistochemical staining with anti‐c‐ myc monoclonal antibody (mAb) MYC‐1. Positive staining was observed in the cytoplasm, but not in the nucleus in tissues fixed with 10% formalin. On the other hand, positive staining was localized in the nucleus in cryopreserved tissues. Of 34 cryopreserved specimens examined, positive staining with MYC‐1 mAb was observed in 1 of 12 (8.3%) of grade 1 (G1), 12 of 15 (80%) of G2 and 6 of 7 (86%) of G3. Positive staining with Ki‐67 mAb was observed in 2 of 12 (17%) of G1, 12 of 15 (80%) of G2, and 6 of 7 (86%) of G3. These results suggest that tumors with higher nuclear pleomorphism contain more proliferating cells.