Open AccessMechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
Author(s) -
Umemura Shinichiro,
Yumita Nagahiko,
Nishigaki Ryuichiro,
Umemura Koshiro
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02674.x
Subject(s) - hematoporphyrin , singlet oxygen , chemistry , oxygen , biophysics , cell damage , cell , photochemistry , biochemistry , biology , photodynamic therapy , organic chemistry
The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematoporphyrin was suppressed by adding histidine but not by mannitol. The enhancement was doubled in rate by substitution of deuterium oxide medium for normal water. Sonoluminescence was produced in a saline solution under similar acoustic conditions and observed to have spectral components that can excite hematoporphyrin molecules. These results suggest that cell damage enhancement is probably mediated via singlet oxygen generated by ultrasonically activated hematoporphyrin.