Open AccessNeoplastic Alteration of a Membrane‐associated Sialidase of Rat Liver
Author(s) -
Miyagi Taeko,
Konno Kimio,
Sagawa Junji,
Tsuiki Shigeru
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02667.x
Subject(s) - sialidase , antiserum , biochemistry , neuraminidase , enzyme , membrane , biology , immunoprecipitation , chemistry , antibody , immunology , gene
Rat liver participate fraction contains two types of membrane‐associated and gangliosides‐hydrolyzing sialidase, which have been shown to be identical to two membrane‐associated sialidases of rat brain (I and II) chromatographically, immunologically and in substrate specificity. Chromatography on AH‐Sepharose 4B of the membrane sialidases of rat primary hepatoma induced by 3′‐methyl‐4‐dimethylaminoazobenzene (MeDAB) further revealed that hepatocarcinogenesis induces a marked decrease in sialidase II but no decrease in sialidase I. Using antisera against sialidases I and II of rat brain, immunoprecipitation studies of the solubilized particulate fractions of rat liver and MeDAB‐hepatoma gave results similar to those obtained chromatographically. Using the same immunological technique, sialidase II but not sialidase I was found to be decreased in AH109 A hepatoma and in regenerating and fetal liver.