Open AccessInhibition of Proliferation and Induction of Differentiation of Human and Mouse Myeloid Leukemia Cells by New Ethyleneglycol‐type Nonphosphorus Alkyl Ether Lipids
Author(s) -
Kasukabe Takashi,
Honma Yoshio,
Hozumi Motoo,
Nomura Hiroaki
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02649.x
Subject(s) - ether , myeloid leukemia , chemistry , cellular differentiation , alkoxy group , leukemia , phospholipid , alkyl , cell growth , myeloid , growth inhibition , biochemistry , cell culture , microbiology and biotechnology , stereochemistry , biology , immunology , organic chemistry , membrane , gene , genetics
A variety of ethyleneglycol‐type nonphosphorus alkyl ether lipids, ether derivatives of diethylene‐glycol in which the two hydroxyl groups were substituted with long chain alkyl and quaternary ammonioalkyl groups, were synthesized and their effects on proliferation and differentiation of cultured human (HL‐60) and mouse (M1) myeloid leukemia cells were studied. Incubation with these compounds inhibited the cellular proliferation, and the cells differentiated into morphologically and functionally mature granulocytes. Of the compounds tested, 1‐[2‐[2‐(octadecyloxy)ethoxy]ethoxy]‐butylpyridinium mesylate (EG‐6) was the most effective in inducing differentiation of HL‐60 cells. Almost maximal induction of differentiation and inhibition of growth of HL‐60 cells on day 6 were observed when the cells were treated with EG‐6 for 1 day and then cultured without EG‐6 for a further 5 days. The inhibitory effect of EG‐6 on the leukemic cells was over 100 times more than that of 2‐[2‐(dodecyloxy)ethoxy]ethyl 2‐pyridinioethyl phosphate, a potent antileukemic ether phospholipid.