Open AccessSpecialized Localization of P‐Glycoprotein Recognized by MRK 16 Monoclonal Antibody in Endothelial Cells of the Brain and the Spinal Cord
Author(s) -
Sugawara Isamu,
Hamada Hirofumi,
Tsuruo Takashi,
Mori Shigeo
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02636.x
Subject(s) - monoclonal antibody , blood–brain barrier , spinal cord , ultrastructure , glycoprotein , central nervous system , biology , endothelial stem cell , antibody , pathology , p glycoprotein , perivascular space , microbiology and biotechnology , immunology , neuroscience , anatomy , medicine , biochemistry , in vitro , antibiotics , multiple drug resistance
This communication describes the cellular and ultrastructural localization in the central nervous system of P‐glycoprotein (P‐GP) recognized by a murine monoclonal antibody, MRK 16. At the ultrastructural level P‐GP was strictly confined to the luminal surface of the endothelial cells which comprise the capillary vessels of the brain and the spinal cord. No P‐GP was found in the endothelial cells of other organs. Our findings may be useful as a means to define the blood‐brain barrier, and they imply that the blood‐brain barrier is anatomically characterized by the presence of intercellular tight junctions between continuous nonfenestrated endothelial cells.