Open AccessGenotypic Differentiation of Intrahepatically Transplanted Hyperplastic Nodule Cells of Analbuminemic and Normal Rat Origin by Polymerase Chain Reaction
Author(s) -
Nishikawa Yuji,
Sakai Hirotsuka,
Inagaki Mitsuhiro,
Fukuda Ikue,
Ogawa Katsuhiro,
Nagase Sumi
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02632.x
Subject(s) - polymerase chain reaction , genotype , nodule (geology) , pathology , biology , microbiology and biotechnology , chemistry , gene , medicine , genetics , paleontology
DNA fragments encompassing the region of the seven‐base‐pair deletion in the albumin gene, which is a characteristic abnormality of Nagase's analbuminemic rat (NAR), were amplified by polymerase chain reaction, and we could differentiate the genotypes of normal rat, homozygous NAR and heterozygous NAR electrophoretically. This genotyping method was applied to the differentiation of hyperplastic nodules on immunostained tissue sections in the intrahepatic transplantation model. When the hyperplastic nodule cells of normal rat were transplanted to the livers of homozygous NAR, the donor cells were effectively differentiated from the host cells by the genotype.