Open AccessColony‐forming Ability in vitro and Clonology of Colorectal Cancer
Author(s) -
Tanigawa Nobuhiko,
Morimoto Hideki
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02628.x
Subject(s) - clonogenic assay , pathology , stroma , colorectal cancer , tumor progression , primary tumor , cancer , medicine , in vitro , cancer research , biology , metastasis , immunohistochemistry , biochemistry
One hundred and twenty‐three specimens (83 primary and 40 metastatic) of colorectal cancers from 102 patients were subjected to the human tumor clonogenic assay (HTCA) to determine the clonogenicities of tumor specimens stratified by the degree of histological differentiation and of tumor progression. No statistically significant differences in the clonogenicity were observed among the tumors with different histological differentiation. Viable malignant cells were abundant in the primary tumor specimens with submucosal (sm) and muscular layer (pm) invasion, and decreased significantly in number per gram of wet specimen as the tumors progressed ( P <0.05). Clonogenicity was also higher in “smd” and “pm” primary tumor specimens, and decreased as the tumors advanced ( P <0.05). These findings suggest that, at the onset or in the relatively early phase of the tumor progression, tumors are medullary and have a large fraction of clonogenic cells, and that they then develop more stroma while losing the clonogenic cell fraction by tumor progression. HTCA appears to be still valuable for analysis of the changes in biological properties over time in large bowel tumors.