Open AccessImmunohistological and Biochemical Detection of Fucosylated Polylactosamine Antigen with Monoclonal Antibody ACFH18 in Gastric Cancer and Normal Gastric Mucosa
Author(s) -
Dohi Taeko,
Abe Kazuo,
Ohshiba Saburo,
Yamaguchi Kenji,
Oshima Mieko
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02626.x
Subject(s) - glycolipid , antigen , monoclonal antibody , epitope , microbiology and biotechnology , immunohistochemistry , cancer , antibody , chemistry , gastric mucosa , biology , pathology , biochemistry , immunology , stomach , medicine , genetics
A mouse monoclonal antibody ACFH18, produced by immunizing with human gastric cell line MKN‐74, recognizes a novel glycolipid antigen, fucosyl‐lactoneodecaosylceramide. In the present study, we investigated the immunohistological localization of this antigen and its biochemical detection in glycolipid fraction in normal and malignant human gastric tissue, compared with other tumor‐associated type‐2 chain glycolipid antigens such as sialyl Le x , sialyl Le x ‐i and Le y . Reactivity with ACFH18 was detected immunohistologically in the proliferating zone of normal fundic gland region as well as in 38 of 54 cases of gastric cancer, with preferential binding to undifferentiated type cancer. Glycolipids reacting with ACFH18, especially slow‐migrating glycolipids on thin‐layer chromatography, were accumulated in all of four cancer specimens compared with normal mucosa obtained from the same patients. Many glycolipids with sialyl Le x or sialyl Le x ‐i epitope were detected in normal mucosa and almost all of these glycolipids were accumulated in cancer specimens. Sialyl Le x ‐i antigen was increased more specifically in cancer than sialyl Le x was. A Le y antigenic glycolipid was considerably decreased in three of four cancer cases and increased in one case. From these results, glycolipid detected by ACFH18 appeared to be a tumor‐associated antigen comparable to sialyl Le x and sialyl Le x ‐i antigens, but with a unique character as an undifferentiated‐type tumor‐associated antigen.