Open AccessDistribution of Human Granulocyte Colony‐stimulating Factor Receptors on Hematopoietic and Nonhematopoietic Tumor Cell Lines
Author(s) -
Kuwaki Tomoaki,
Hosoi Takayuki,
Hanazono Yutaka,
Tsumura Haruhiko,
Ishikawa Fuyuki,
Miyazono Kohei,
Miyagawa Kiyoshi,
Takaku Fumimaro
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02608.x
Subject(s) - haematopoiesis , granulocyte , granulocyte colony stimulating factor , distribution (mathematics) , receptor , biology , hematopoietic cell , cell culture , cancer research , immunology , microbiology and biotechnology , pathology , medicine , stem cell , genetics , chemotherapy , mathematical analysis , mathematics
of two tyrosine residues into human granulocyte colony‐stimulating factor (G‐CSF) allowed us to obtain radioiodinated material efficiently. By using this material, specific receptors for human G‐CSF were analyzed. It was found that human G‐CSF receptors were expressed on several myelocytic and monocytic leukemia cell lines. Furthermore, they were also expressed on two chorio‐carcinoma cell lines. However, the human G‐CSF receptors were not detected on other nonhemato‐poietic tumor cell lines examined, indicating that expression of G‐CSF receptors is strictly limited as compared with receptors for granulocyte/macrophage colony‐stimulating factor (GM‐CSF), which are widely distributed on nonhematopoietic tumors.