Open AccessAcquired Expression of hst‐1 in an Autonomous Subline (Chiba Subline 2) Derived from Androgen‐responsive Mouse Mammary Tumor (Shionogi Carcinoma 115)
Author(s) -
Akakura Koichiro,
Furuya Yuzo,
Sato Naohide,
Kodama Takaomi,
Teshima Shinichi,
Shimosato Yukio,
Yoshida Teruhiko,
Terada Masaaki,
Shimazaki Jun
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02606.x
Subject(s) - mouse mammary tumor virus , biology , androgen , mammary tumor , mammary gland , cell culture , endocrinology , medicine , testosterone (patch) , microbiology and biotechnology , cancer research , dna , cancer , hormone , genetics , breast cancer
Since growth of Shionogi Carcinoma 115 (SC 115) and its autonomous subline (CS 2) were regulated by fibroblast growth factor‐like peptide, expression of int‐2 and hst‐1 was examined in these cell lines. Hybridization of genomic DNA with long terminal repeat of mouse mammary tumor virus (MMTV) revealed the same pattern of restriction fragments, showing the same integration of MMTV. Although weak expression of int‐2 was noticed in the two cells, clear expression of hst‐1 was seen only in CS 2 cultured with/without testosterone. It is suggested that autonomous growth of androgen‐unresponsive CS 2 is connected with expression of hst‐1 .