Open AccessA Domain Responsible for the Transformation Suppressor Activity in K rev ‐1 Protein
Author(s) -
Kitayama Hitoshi,
Matsuzaki Tomoko,
Ikawa Yoji,
Noda Makoto
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02589.x
Subject(s) - suppressor , effector , complementary dna , transformation (genetics) , biology , gene , fusion protein , microbiology and biotechnology , chimeric gene , genetics , recombinant dna , gene expression
K rev ‐1 cDNA encodes a ras ‐related protein and exhibits an activity of inducing flat revertants at certain frequencies (2‐5% of total transfectants) when introduced into a v‐K‐ ras ‐transformed mouse NIH3T3 cell line, DT. To explore the functional organization of K rev ‐1 protein, we constructed a series of chimeric genes consisting of fragments of H‐ ras and K rev ‐1 cDNAs, and tested their biological activities in DT cells. The results indicated that the determinant for the transformation suppressor activity resides in the N‐terminal one‐third of the K rev ‐1 encoded polypeptide within which a highly conserved, putative effector‐binding domain is present.