Open AccessMutational Activation of H‐ ras and K‐ ras Genes Is Absent in N ‐Nitroso‐ N ‐methylurea‐induced Liver Tumors in Rats
Author(s) -
Sakai Hirotsuka,
Ogawa Katsuhiro
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02587.x
Subject(s) - gene , microbiology and biotechnology , nitroso , biology , chemistry , genetics , cancer research , medicinal chemistry
We examined mutational activation of H‐ and K‐ ras genes in hyperplastic nodules and hepatocellular carcinomas induced by N ‐nitroso‐ N ‐methylurea or diethylnitrosamine using the polymerase chain reaction, followed by dot‐blot hybridization. No mutational activation was detected in these hepatic lesions. The results indicate that low incidence of the activation of H‐ and K‐ ras genes in rat liver tumors is due to the organ specificity rather than the nature of the carcinogens used.