Open AccessA Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
Author(s) -
Harada Fumio,
Hirose Yutaka,
Tanaka Motohiro,
Sasaki Takuma,
Kamiya Hiroyuki,
Miura Kazunobu
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02555.x
Subject(s) - subgenomic mrna , oligonucleotide , biology , microbiology and biotechnology , murine leukemia virus , rna , virology , virus , microrna , cancer cell , cell culture , leukemia , nucleic acid , cancer , gene , genetics
A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A + RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA Pro ‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.