Open AccessHeterogeneity of Antigen Molecules Recognized by Anti‐tax 1 Monoclonal Antibody Lt‐4 in Cell Lines Bearing Human T Cell Leukemia Virus Type I and Related Retroviruses
Author(s) -
Tanaka Yuetsu,
Yoshida Atsushi,
Takayama Yoshinaga,
Tsujimoto Hajime,
Tsujimoto Atsumi,
Hayami Masanori,
Tozawa Hideki
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02554.x
Subject(s) - antigen , cell culture , monoclonal antibody , biology , immunofluorescence , virology , microbiology and biotechnology , t cell leukemia , virus , leukemia , cell , antibody , immunology , biochemistry , genetics
Using a monoclonal antibody, Lt‐4, directed against human T cell leukemia virus type I (HTLV‐I) trans ‐activator (tax 1 ) antigen, we examined the expression of tax 1 and related antigens in a variety of T cell lines bearing HTLV‐I and related retroviruses, simian T cell leukemia virus type I (STLV‐I) and HTLV‐II, by immunofluorescence and immunoblot assays. Lt‐4 reacted with all HTLV‐I‐bearing cell lines tested and five out of eight simian cell lines bearing STLV‐I, but not with an HTLV‐II‐bearing cell line. Lt‐4 detected 40 kd tax 1 antigen molecules in most HTLV‐I‐bearing cell lines except one cell line that expressed 39 kd tax 1 antigen. In the STLV‐I‐bearing T cell lines, tax 1 ‐related antigen molecules detected by Lt‐4 were heterogeneous, having molecular weights in the range of 36–41 kd.