Open AccessEffect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
Author(s) -
Routledge Michael N.,
Orton Terry C.,
Lord Peter G.,
Garner R. Colin
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02553.x
Subject(s) - butylated hydroxyanisole , corn oil , aristolochic acid , chemistry , adduct , biochemistry , food science , biology , antioxidant , organic chemistry , genetics
Administration of butylated hydroxyanisole (BHA) orally at either 0.5 g or 1 g/kg daily for 14 days to rats did not produce any DNA adducts in the forestomach as measured by the 32 P‐postlabeling method using (1) limiting concentrations of 32 P‐ATP; (2) nuclease P 1 enhancement; or (3) butanol extraction. Experiments were conducted to establish the effects of BHA administration on aristolochic acid (AA) DNA adduct formation in the forestomach and liver, when BHA was administered prior to, together with or after AA administration. Adduct levels per 10 9 nucleotides in the liver after oral dosing daily for 5 days with 1 mg/kg AA and BHA (1 g/kg) or corn oil (5 ml/kg) for 7 days were as follows: (a) BHA and AA given simultaneously; 235±71, (b) AA+corn oil; 63±39, (c) AA followed by BHA; 57±13, (d) AA followed by corn oil; 91±38, (e) BHA followed by AA; 90±12, (f) corn oil followed by AA; 83±24. For the forestomach the values were: (a) 236±86, (b) 77±25, (c) 367±97, (d) 296±47, (e) 217±81, (f) 70±64. These data suggest that BHA could have an enhancing effect on AA‐induced lesions in the forestomach if dosed together with, or prior to, AA as adduct levels are significantly higher than in controls.