Open AccessEnhancement of the Antibody‐dependent Tumoricidal Activity of Human Monocytes by Human Monocytic Colony‐stimulating Factor
Author(s) -
Suzu Shinya,
Yanai Nobuya,
Saito Minoru,
Kawashima Takuji,
Kasahara Tadashi,
Saito Masaki,
Takaku Fumimaro,
Motoyoshi Kazuo
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02510.x
Subject(s) - antibody dependent cell mediated cytotoxicity , monocyte , antibody , cell culture , cytotoxicity , receptor , microbiology and biotechnology , biology , fc receptor , colony stimulating factor , macrophage colony stimulating factor , immunology , chemistry , monoclonal antibody , macrophage , haematopoiesis , in vitro , stem cell , biochemistry , genetics
Human monocytic colony‐stimulating factor (hM‐CSF) stimulates mature cells as well as progenitor cells of human monocyte lineage. In the present study, we investigated the effect of completely purified native hM‐CSF on the tumoricidal activity of human monocytes against Raji (Burkitt lymphoma‐derived cell line) using a chromium release assay. Preincubation of human peripheral blood monocytes with hM‐CSF for 48 h markedly enhanced their antibody‐dependent cellular cytotoxicity (ADCC). This enhancing effect was dependent on the concentration of hM‐CSF, and maximal enhancement was achieved at the concentration of 66 ng/ml. The number of receptor sites on monocytes against the Fc portion of immunoglobulin G increased 2‐ to 4‐fold during 48 h incubation with hM‐CSF. These results suggest that hM‐CSF stimulates monocyte‐ADCC by increasing the number of Fc receptor sites on human monocytes.